Glucose Oxidase is an oxidoreductase molecule. The enzyme, which has a molecular weight of 192.000 Dalton, is mainly available from microbial sources and is normally produced by the controlled fermentation af Aspergillus niger or Penicillium amagaskinense.
Glucose Oxidase has no activity itself, but in presence of specific substrates, the enzyme is used to scavenge either glucose or oxygen. The products of the reaction are Gluconic Acid and hydrogen Peroxide.
The reaction is optimal between 30 to 50°C and at pH between 4.5 to 6.5.